2002 - 2006 B.E., Department of Optical Engineering, Zhejiang University

2006 - 2011 Ph.D., Department of Electronic & Computer Engineering, Hong Kong University of Science & Technology

2011 - 2015 Postdoctoral Associate, Janelia Research Campus, Howard Hughes Medical Institute

2015 - Professor, Institute of Biophysics, Chinese Academy of Science

2021, National Science Fund for Distinguished Young Scholar, China

2019, XPLORER prize, Tencent Foundation

2019, VCANBIO award for innovations and breakthroughs in life sciences and medicine

2015, PicoQuant Young Investigator Award, SPIE

2011, Winner of Young Scientist Award, Hong Kong Institute of Science

We focus on developing novel optical imaging methods, which enable us to measure and study the biological processes with unprecedented precision, such as structures and dynamics of molecular assemblies in living biological systems, interactions between molecules, and behavior of individual molecules, etc. We are greatly enjoying working at the interface of several disciplines, where light, biology and chemistry catalyze each other.

1. Qiao C^#, Li D^#, Liu Y^#, Zhang S^#, Liu K, Liu C, Guo Y, Jiang T, Fang C, Li N, Zeng Y, He K, Zhu X, Lippincott-Schwartz J*, Dai Q*,Li D*. Rationalized deep learning super-resolution microscopy for sustained live imaging of rapid subcellular processes.Nature Biotechnology, Published: 06 Oct 2022.

2. Andrews B, Chang J, Collinson L,Li D, Lundberg E, Mahamid J, Manley S, Mhlanga M, Nakano A, Sch?neberg J, Valen D, Wu T, Zaritsky A. Imaging cell biology.Nature Cell Biology, 2022 Aug; 24(8):1180-1185. (invited Viewpoint paper)

3. Jiang A^#, Zhang S^#, Wang X*,Li D*. RBM15 modulates STYK1 m6A to promote tumorigenesis.Comput Struct Biotechnol J, 2022 Sep 5;20:4825-4836.

4. Gong B^#, Guo Y^#, Ding S, Liu X, Meng A,Li D*, Jia S*, A Golgi-derived vesicle potentiates PtdIns4P to PtdIns3P conversion for endosome fission.Nature Cell Biology,2021Jul; 23(7):782-795.

5. Xu G^#, Liu C^#, Zhou S^#, Li Q, Feng Y, Sun P, Feng H, Gao Y, Zhu J, Luo X, Zhan Q, Liu S, Zhu S, Deng H*,Li D*, Gao P*. Viral tegument proteins restrict cGAS-DNA phase separation to mediate immune evasion.Molecular Cell,2021Jul 1;81(13):2823-2837.

6. Li D^#, Colin-York H^#, Barbieri L^#, Javanmardi Y^#, Guo Y, Korobchevskaya K, Moeendarbary E*,Li D*, Fritzsche M*. Astigmatic traction force microscopy (aTFM).Nature Communications,2021Apr 12; 12(1):2168.

7. Barbieri L^#, Colin-York H^#, Korobchevskaya K^#, Li D, Wolfson DL, Karedla N, Schneider F, Ahluwalia BS, Seternes T, Dalmo RA, Dustin ML,Li D*, Fritzsche M*. Two-dimensional TIRF-SIM-traction force microscopy (2D TIRF-SIM-TFM).Nature Communications,2021Apr 12;12(1):2169.

8. Qiao C^#, Chen X^#, Zhang S^#, Li D, Guo Y, Dai Q*,Li D*. 3D Structured Illumination Microscopy via Channel Attention Generative Adversarial Network.IEEE J SEL TOP QUANT.2021Feb 22;27(4):1-11.

9. Qiao C^#, Li D^#, Guo Y^#, Liu C^#, Jiang T, Dai Q*,Li D*. Evaluation and development of deep neural networks for image super-resolution in optical microscopy.Nature Methods,2021Feb;18(2):194-202.

10. Wang X^#*, Liu C^#, Zhang S^#, Yan H, Zhang L, Jiang A, Liu Y, Feng Y, Li D, Guo Y, Hu X, Lin Y, Bu P,Li D*. N⁶-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles.Developmental Cell,2021Mar 8;56(5):702-715.

11. Qin J, Guo Y, Xue B, Shi P, Chen Y, Su Q, Hao H, Zhao S, Wu C, Yu L,Li D*, Sun Y*. ER-mitochondria contacts promote mtDNA nucleoids active transportation via mitochondrial dynamic tubulation.Nature Communications,2020Sep 8;11(1):4471.

12. Li D^#, Zhao Y^#, Li D, Zhao H, Huang J, Miao G, Feng D, Liu P,Li D*, Zhang H *. The ER-Localized Protein DFCP1 Modulates ER-Lipid Droplet Contact Formation.Cell Reports,2019Apr 9;27(2):343-358.

13. Colin-York H^#, Javanmardi Y^#, Barbieri L, Li D, Korobchevskaya K, Guo Y, Hall C, Taylor A, Khuon S, Sheridan G, Chew T,Li D*, Moeendarbary E*, Fritzsche M*. Spatiotemporally Super-Resolved Volumetric Traction Force Microscopy.Nano Letters,2019Jul 10;19(7):4427-4434.

14. Guo Y^#, Li D^#, Zhang S, Yang Y, Liu JJ, Wang X, Liu C, Milkie DE, Moore RP, Tulu US, Kiehart DP, Hu J, Lippincott-Schwartz J*, Betzig E*,Li D*. Visualizing intracellular organelle and cytoskeletal interactions at nanoscale resolution on millisecond timescales.Cell,2018Nov 15;175(5):1430-1442.

15. Han X^#, Li P^#, Yang Z, Huang X, Wei G, Sun Y, Kang X, Hu X, Deng Q, Chen L, He A, Huo Y,Li D*, Betzig E, Luo J*. Zyxin regulates endothelial von Willebrand factor secretion by reorganizing actin filaments around exocytic granules.Nature Communications,2017Mar 3; 8:14639.

16.Li D, Shao L, Chen BC, Zhang X, Zhang M, Moses B, Milkie DE, Beach JR, Hammer JA, Pasham M, Kirchhausen T, Baird MA, Davidson MW, Xu P, Betzig E*. Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics.Science,2015Aug 28;349(6251): aab3500.Cover article.

17. Beach JR*, Shao L, Remmert K,Li D, Betzig E, Hammer JA*. Nonmuscle myosin II isoforms coassemble in living cells.Current Biology,2014May 19;24(10):1160-1166.

18.Li D^#, Zheng W^#, Zeng Y, Luo Y, Qu JY*. Two-photon excited hemoglobin fluorescence provides contrast mechanism for label-free imaging of microvasculature in vivo.Optics Letters,2011Mar 15;36(6):834-836.

19.Li D^#, heng W^#, Zhang W, Teh SK, Zeng Y, Luo Y, Qu JY*. Time-resolved detection enables standard two-photon fluorescence microscope for in vivo label-free imaging of microvasculature in tissue.Optics Letters,2011Jul 15;36(14):2638-2640.

20.Li D, Zheng W, Zeng Y, Qu JY*. In vivo and simultaneous multimodal imaging: integrated multiplex coherent anti-Stokes Raman scattering and two-photon microscopy.Applied Physics Letters,201097(22): 223702.

21.Li D, Zheng W, Qu JY*. Imaging of epithelial tissue in vivo based on excitation of multiple endogenous nonlinear optical signals.Optics Letters,2009Sep 15;34(18):2853-2855.

22.Li D, Zheng W, Qu JY*. Two-photon autofluorescence microscopy of multicolor excitation.Optics Letters,2009Jan 15;34(2):202-204.

23.Li D, Zheng W, Qu JY*. Time-resolved spectroscopic imaging reveals the fundamentals of cellular NADH fluorescence.Optics Letters,2008Oct 15;33(20):2365-2367.

(From Dong Li, January 12, 2023)