Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein,Nano letters, 02 Oct 2019
Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein
Mingshu Zhang, Zhifei Fu, Changqing Li, Anyuan Liu, Dingming Peng, Fudong Xue, Wenting He, Shan Gao, Fan Xu, Dan Xu, Ling Yuan, Fa Zhang, Zhiheng Xu, Tao Xu* and Pingyong Xu*
Abstract
Low temporal resolution and limited photocontrollable fluorescent protein probes have restricted the widespread application of single-molecule localization microscopy (SMLM). In the current study, we developed a new photoconvertible fluorescent protein (PCFP), pcStar, and quick single molecule-guided Bayesian localization microscopy (Quick-SIMBA). The combination of pcStar and Quick-SIMBA achieved the highest temporal resolution (0.1–0.25 s) with large field-of-view (76 × 9.4 μm2?76 × 31.4 μm2) among the SMLM methods, which enabled the dynamic movements of the endoplasmic reticulum dense tubular matrix to be resolved. Moreover, pcStar extended the application of SMLM to imaging the immediate early nanostructures inDrosophilaembryos and revealed a specific “parallel three-pillar” structure in the neuronal-glial cell junction, helping to elucidate glial cell “locking” and support of neurons duringDrosophilaembryogenesis.
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Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein,Nano letters, 02 Oct 2019
Nano letters, 02 October, 2019,DOI:https://doi.org/10.1021/acs.nanolett.9b02855
Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein
Mingshu Zhang, Zhifei Fu, Changqing Li, Anyuan Liu, Dingming Peng, Fudong Xue, Wenting He, Shan Gao, Fan Xu, Dan Xu, Ling Yuan, Fa Zhang, Zhiheng Xu, Tao Xu* and Pingyong Xu*
Abstract
Low temporal resolution and limited photocontrollable fluorescent protein probes have restricted the widespread application of single-molecule localization microscopy (SMLM). In the current study, we developed a new photoconvertible fluorescent protein (PCFP), pcStar, and quick single molecule-guided Bayesian localization microscopy (Quick-SIMBA). The combination of pcStar and Quick-SIMBA achieved the highest temporal resolution (0.1–0.25 s) with large field-of-view (76 × 9.4 μm2?76 × 31.4 μm2) among the SMLM methods, which enabled the dynamic movements of the endoplasmic reticulum dense tubular matrix to be resolved. Moreover, pcStar extended the application of SMLM to imaging the immediate early nanostructures inDrosophilaembryos and revealed a specific “parallel three-pillar” structure in the neuronal-glial cell junction, helping to elucidate glial cell “locking” and support of neurons duringDrosophilaembryogenesis.
文章链接:https://pubs.acs.org/doi/10.1021/acs.nanolett.9b02855
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